RT Journal Article
SR Electronic
T1 Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a Light-Responsive Polymer
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 280487
DO 10.1101/280487
A1 Yohei Hayashi
A1 Junichi Matsumoto
A1 Shohei Kumagai
A1 Kana Morishita
A1 Long Xiang
A1 Yohei Kobori
A1 Seiji Hori
A1 Masami Suzuki
A1 Toshiyuki Kanamori
A1 Kazuhiro Hotta
A1 Kimio Sumaru
YR 2018
UL http://biorxiv.org/content/early/2018/03/12/280487.abstract
AB Conventional cell handling and sorting methods require manual dissociation, which decreases cell quality and quantity and is not well suited for monitoring applications. To purify adherent cultured cells, in situ cell purification technologies that are high throughput and can be utilized in an on-demand manner are expected. Previous demonstrations using direct laser-mediated cell elimination revealed only limited success in terms of their usability and throughput. Here, we developed a Laser-Induced, Light-responsive-polymer-Activated, Cell Killing (LILACK) system that enables high-speed and on-demand adherent cell sectioning and purification. This system employs a visible laser beam, which does not kill cells directly, but induces local heat production through the trans-cis-trans photo-isomerization of azobenzene moieties in only the irradiated area of a light-responsive thin layer. Using this system in each passage for sectioning, human induced pluripotent stem cells (hiPSCs) were maintained their pluripotency and self-renewal during long-term culture. Furthermore, combined with deep machine-learning analysis on fluorescent and phase contrast images, a label-free and automatic cell processing system was developed by eliminating unwanted spontaneously differentiated cells in undifferentiated hiPSC culture conditions.