RT Journal Article
SR Electronic
T1 Aneuploidy promotes intestinal dysplasia in Drosophila
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 280768
DO 10.1101/280768
A1 Luís Pedro Resende
A1 Augusta Monteiro
A1 Rita Brás
A1 Tatiana Lopes
A1 Claudio E. Sunkel
YR 2018
UL http://biorxiv.org/content/early/2018/03/12/280768.abstract
AB Aneuploidy is associated with different human diseases, particularly cancer, but how different cell types within tissues respond to aneuploidy is not fully understood. In some studies, aneuploidy has been shown to have a deleterious effect and lead to cell death, however it has also been shown to be a causal event of tumorigenesis in other contexts.Here, we show that Drosophila intestinal stem cells have a particular tolerance to aneuploidy and do not activate apoptosis in response to chromosome misegregation like other non-stem cells. Instead, we observe the development of tissue dysplasia characterized by an accumulation of progenitor cells, increased stem cell proliferation rate, and an excess of cells of the enteroendocrine lineage. Our findings highlight the importance of mechanisms acting to prevent aneuploidy within tissue stem cells and provide an in vivo model of how these cells can act as reservoirs for genomic alterations that can lead to dysplasia.armarmadillocenp-metacentromeric protein metaEBenteroblastECenterocyteEEenteroendocrine Esg - escargotGFPgreen fluorescent proteinISCintestinal stem cellpH3phospho-histone 3 pros - prosperoSACspindle-assembly checkpointSaKsak kinaseSu(H)supressor of hairlessYFPyellow fluorescent protein