RT Journal Article
SR Electronic
T1 Endosomal explosion induced by hypertonicity enhances chitosan transfection
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 730648
DO 10.1101/730648
A1 Shupeng Wang
A1 Shaohua Jin
A1 Guangzhi Li
A1 Rui Sun
A1 Qinghai Shu
A1 Song Wu
YR 2019
UL http://biorxiv.org/content/early/2019/08/09/730648.abstract
AB Transferring DNA into cells to regulate cell function is a novel research field in recent decades. Chitosan is a gene vector with the properties of low-cost and safe, but high efficient delivery has remained challenging. We developed a strategy termed EEIH, for endosomal explosion induced by hypertonicity, in which short-time exposure to hypertonic solutions triggers endosomes destabilization like explosions. EEIH can force chitosan/DNA polyplexes to break through the endosomal barriers to approach the nucleus, which results in boosting the transfection efficiency of chitosan in several cell lines. We demonstrate that EEIH is a significant and practical strategy in chitosan transfection system without sophisticated modification of chitosan.