PT  - JOURNAL ARTICLE
AU  - Patrick M. Helbling
AU  - Elena Piñeiro-Yáñez
AU  - Rahel Gerosa
AU  - Steffen Boettcher
AU  - Fátima Al-Shahrour
AU  - Markus G. Manz
AU  - César Nombela-Arrieta
TI  - Global transcriptomic profiling of the bone marrow stromal microenvironment during postnatal development, aging and inflammation
AID  - 10.1101/730457
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 730457
4099  - http://biorxiv.org/content/early/2019/08/09/730457.short
4100  - http://biorxiv.org/content/early/2019/08/09/730457.full
AB  - Bone marrow (BM) stromal cells provide the structural and regulatory framework for hematopoiesis and contribute to developmental-stage specific niches, such as those preserving hematopoietic stem cell (HSCs). Despite recent advances in our understanding of stromal composition and function, little is known on the dynamic transcriptional remodeling that this compartment undergoes over time and during adaptation to stress. Similarly, how molecular changes in stroma are linked to age-related modulation of hematopoiesis is poorly understood. Using RNA-sequencing, we performed a longitudinal comparison of the transcriptional profile of four principal mesenchymal and endothelial stromal subsets, namely CXCL12-abundant reticular (CARc), PDGFR-α+Sca-1+, sinusoidal (SECs) and arterial endothelial cells (AECs), isolated from early postnatal, adult and aged mice. Our data i) provide molecular fingerprints defining novel, cell-specific anatomical and functional features ii) reveal radical reprogramming of pro-hematopoietic, immune and matrisomic transcriptional programs during the narrow temporal transition from juvenile to adult stages iii) demonstrate that homeostatic aging is characterized by a progressive and pronounced upregulation of pro-inflammatory gene-expression and loss of stromal cell fitness. By profiling in vivo responses of stromal cells to infection-mimicking agents, we finally demonstrate that transcriptomic pathways elicited by sterile inflammation are largely recapitulated during aging, thereby supporting the inflammatory basis of aging-related adaptations of BM hematopoietic function.