PT  - JOURNAL ARTICLE
AU  - Vahid Omidvar
AU  - Sheshanka Dugyala
AU  - Feng Li
AU  - Susan Rottschaefer
AU  - Marisa E. Miller
AU  - Mick Ayliffe
AU  - Matthew J. Moscou
AU  - Shahryar F. Kianian
AU  - Melania Figueroa
TI  - Detection of race-specific resistance against <em>Puccinia coronata</em> f. sp. <em>avenae</em> in <em>Brachypodium</em> species
AID  - 10.1101/281568
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 281568
4099  - http://biorxiv.org/content/early/2018/03/13/281568.1.short
4100  - http://biorxiv.org/content/early/2018/03/13/281568.1.full
AB  - Oat crown rust caused by Puccinia coronata f. sp. avenae is the most destructive foliar disease of cultivated oat. Characterization of genetic factors controlling resistance responses to Puccinia coronata f. sp. avenae in non-host species could provide new resources for developing disease protection strategies in oat. We examined symptom development and fungal colonization levels of a collection of Brachypodium distachyon and B. hybridum accessions infected with three North American P. coronata f. sp. avenae isolates. Our results demonstrated that resistance phenotypes are dependent on both host and pathogen genotypes, indicating a role for race-specific responses in these interactions. These responses were independent of the accumulation of reactive oxygen species. Expression analysis of several defense-related genes suggested that salicylic acid and ethylene-mediated signaling, but not jasmonic acid are components of resistance reaction to P. coronata f. sp. avenae. Our findings suggest that effector-triggered immunity contributes to non-host resistance to P. coronata f. sp. avenae in Brachypodium species and provide the basis to conduct a genetic inheritance study to examine this hypothesis.