%0 Journal Article %A Jason E. Chung %A Hannah R. Joo %A Jiang Lan Fan %A Daniel F. Liu %A Alex H. Barnett %A Supin Chen %A Charlotte Geaghan-Breiner %A Mattias P. Karlsson %A Magnus Karlsson %A Kye Y. Lee %A Hexin Liang %A Jeremy F. Magland %A W. Hamish Mehaffey %A Angela C. Tooker %A Michael S. Brainard %A Leslie F. Greengard %A Vanessa M. Tolosa %A Loren M. Frank %T High-density, long-lasting, and multi-region electrophysiological recordings using polymer electrode arrays %D 2018 %R 10.1101/242693 %J bioRxiv %P 242693 %X The brain is a massive neuronal network, organized into anatomically distributed sub-circuits, with functionally relevant activity occurring at timescales ranging from milliseconds to months. Current methods to monitor neural activity, however, lack the necessary conjunction of anatomical spatial coverage, temporal resolution, and long-term stability to measure this distributed activity. Here we introduce a large-scale, multi-site recording platform that integrates polymer electrodes with a modular stacking headstage design supporting up to 1024 recording channels in freely behaving rats. This system can support months-long recordings from hundreds of well-isolated units across multiple brain regions. Moreover, these recordings are stable enough to track 25% of single units for over a week. We also demonstrate long-lasting, single-unit recordings in songbird. This platform enables large-scale electrophysiological interrogation of the fast dynamics and long-timescale evolution of anatomically distributed circuits, and thereby provides a new tool for understanding brain activity. %U https://www.biorxiv.org/content/biorxiv/early/2018/03/13/242693.full.pdf