RT Journal Article SR Electronic T1 High-density, long-lasting, and multi-region electrophysiological recordings using polymer electrode arrays JF bioRxiv FD Cold Spring Harbor Laboratory SP 242693 DO 10.1101/242693 A1 Jason E. Chung A1 Hannah R. Joo A1 Jiang Lan Fan A1 Daniel F. Liu A1 Alex H. Barnett A1 Supin Chen A1 Charlotte Geaghan-Breiner A1 Mattias P. Karlsson A1 Magnus Karlsson A1 Kye Y. Lee A1 Hexin Liang A1 Jeremy F. Magland A1 W. Hamish Mehaffey A1 Angela C. Tooker A1 Michael S. Brainard A1 Leslie F. Greengard A1 Vanessa M. Tolosa A1 Loren M. Frank YR 2018 UL http://biorxiv.org/content/early/2018/03/13/242693.abstract AB The brain is a massive neuronal network, organized into anatomically distributed sub-circuits, with functionally relevant activity occurring at timescales ranging from milliseconds to months. Current methods to monitor neural activity, however, lack the necessary conjunction of anatomical spatial coverage, temporal resolution, and long-term stability to measure this distributed activity. Here we introduce a large-scale, multi-site recording platform that integrates polymer electrodes with a modular stacking headstage design supporting up to 1024 recording channels in freely behaving rats. This system can support months-long recordings from hundreds of well-isolated units across multiple brain regions. Moreover, these recordings are stable enough to track 25% of single units for over a week. We also demonstrate long-lasting, single-unit recordings in songbird. This platform enables large-scale electrophysiological interrogation of the fast dynamics and long-timescale evolution of anatomically distributed circuits, and thereby provides a new tool for understanding brain activity.