TY - JOUR T1 - Cytosine but not adenine base editor generates mutations in mice JF - bioRxiv DO - 10.1101/731927 SP - 731927 AU - Hye Kyung Lee AU - Harold E. Smith AU - Chengyu Liu AU - Michaela Willi AU - Lothar Hennighausen Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/12/731927.abstract N2 - Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms and its ultimate success therapeutically depends on its accuracy. Here we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos using unbiased whole genome sequencing of a family-based trio cohort. We demonstrate that BE4-edited mice carry an excess of single-nucleotide variants and deletions compared to ABE-edited mice and controls. ER -