TY - JOUR T1 - A new twist on bacterial motility – two distinct type IV pili revealed by cryoEM JF - bioRxiv DO - 10.1101/720938 SP - 720938 AU - Alexander Neuhaus AU - Muniyandi Selvaraj AU - Ralf Salzer AU - Julian D. Langer AU - Kerstin Kruse AU - Kelly Sanders AU - Bertram Daum AU - Beate Averhoff AU - Vicki A. M. Gold Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/12/720938.abstract N2 - Many bacteria express flexible protein filaments on their surface that enable a variety of important cellular functions. Type IV pili are examples of such filaments and are comprised of a helical assembly of repeating pilin subunits. Type IV pili are involved in motility (twitching), surface adhesion, biofilm formation and DNA uptake (natural transformation). They are therefore powerful structures that enable bacterial proliferation and genetic adaptation, potentially leading to the development of pathogenicity and antibiotic resistance. They are also targets for drug development.By a complement of experimental approaches, we show that the bacterium Thermus thermophilus produces two different forms of type IV pilus. We have determined the structures of both and built atomic models. The structures answer key unresolved questions regarding the molecular architecture of type IV pili and identify a new type of pilin. We also delineate the roles of the two filaments in promoting twitching and natural transformation. ER -