TY - JOUR T1 - Fascin regulates protrusions and delamination to mediate invasive, collective cell migration <em>in vivo</em> JF - bioRxiv DO - 10.1101/734475 SP - 734475 AU - Maureen C. Lamb AU - Kelsey K. Anliker AU - Tina L. Tootle Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/13/734475.abstract N2 - Fascin is an actin bundling protein that is essential for developmental cell migrations and promotes cancer metastasis. In addition to bundling actin, Fascin has several actin-independent roles. Border cell migration during Drosophila oogenesis provides an excellent model to study Fascin’s various roles during invasive, collective cell migration. Border cell migration requires Fascin. Fascin functions not only within the migrating border cells, but also within the nurse cells, the substrate for this migration. Loss of Fascin results in increased, shorter and mislocalized protrusions during migration. Data supports the model that Fascin promotes the activity of Enabled, an actin elongating factor, to regulate migration. Additionally, loss of Fascin inhibits border cell delamination. These defects are partially due to altered E-cadherin localization in the border cells; this is predicted to be an actin-independent role of Fascin. Overall, Fascin is essential for multiple aspects of this invasive, collective cell migration, and functions in both actin-dependent and -independent manners. These findings have implications beyond Drosophila, as border cell migration has emerged as a model to study mechanisms mediating cancer metastasis. ER -