PT  - JOURNAL ARTICLE
AU  - Jaewoo Hong
AU  - Peng Qu
AU  - Todd R. Wuest
AU  - P. Charles Lin
TI  - Neutrophilic granule protein is a novel murine LPS antagonist
AID  - 10.1101/733352
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 733352
4099  - http://biorxiv.org/content/early/2019/08/13/733352.short
4100  - http://biorxiv.org/content/early/2019/08/13/733352.full
AB  - NGP was previously reported as a granular protein of neutrophils in mouse but the function has not been known clearly. We found the presence of the possible signal peptide in NGP and we hypothesized this protein is being secreted to the blood stream. Since this protein has sequence similarity with an antimicrobial protein cathelicidin, we observed the aspect of inflammation of NGP. Interestingly, NGP interacts with the complex of LPS and LBP and blocked the inflammatory function of NGP. This inhibitory effect of NGP was through the inhibition of LPS-LBP complex to interact with TLR4 molecule. Furthermore, the inhibitory function of NGP could be repeated on the inflammatory effect of LPS in both in vitro and in vivo. With these findings, we report NGP is a novel secretory protein to mask LPS and inhibits its function.