RT Journal Article
SR Electronic
T1 Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 281915
DO 10.1101/281915
A1 Ning Jiang
A1 Can-Jun Ruan
A1 Xiao-Rui Cheng
A1 Lu-Ning Wang
A1 Ji-Ping Tan
A1 Wei-Shan Wang
A1 Fang Liu
A1 Wen-Xia Zhou
A1 Yong-Xiang Zhang
YR 2018
UL http://biorxiv.org/content/early/2018/03/14/281915.abstract
AB Introduction The aim of this study was to investigate the potential role of a microRNA panel as early diagnostic markers for Alzheimer’s disease (AD).Methods The differentially expressed serum microRNAs were screened with microarray among cognitively normal controls (CNC), mild cognitive impairment (MCI), and AD. QRT-PCR assay was applied to evaluate differentially expressed microRNAs with two independent cohorts including 202 participants. Logistic regression model based on microRNA panel was constructed using a training cohort and then validated using an independent cohort.Results First, four differentially expressed serum microRNAs (let-7g, miR-197, miR-126 and miR-29a) were found, which expressions were positively correlated with mini mental state examination (MMSE) score. Second, a microRNA panel with the four microRNAs demonstrated good diagnostic performance for MCI and AD with 84% and 92% accuracy. Third, when combined with MMSE score, the diagnostic performance of the microRNA panel was further improved.Discussion Blood microRNAs are potential AD biomarkers that may lead to new diagnostic strategies.