RT Journal Article SR Electronic T1 Automated sequential chromogenic IHC double staining with two HRP substrates JF bioRxiv FD Cold Spring Harbor Laboratory SP 276501 DO 10.1101/276501 A1 Kenneth H Petersen A1 Jesper Lohse A1 Lasse Ramsgaard YR 2018 UL http://biorxiv.org/content/early/2018/03/14/276501.abstract AB Automated IHC double staining using DAB and HRP Magenta is illustrated utilizing a new acid block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.