@article {Hong282103, author = {Shi-Rong Hong and Cuei-Ling Wang and Yao-Shen Huang and Yu-Chen Chang and Ya-Chu Chang and Ganesh V. Pusapati and Chun-Yu Lin and Ning Hsu and Hsiao-Chi Cheng and Yueh-Chen Chiang and Wei-En Huang and Nathan C. Shaner and Rajat Rohatgi and Takanari Inoue and Yu-Chun Lin}, title = {Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling}, elocation-id = {282103}, year = {2018}, doi = {10.1101/282103}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells and are suggested to be involved in a wide range of cellular activities. However, the complexity and dynamic distribution of tubulin PTMs within cells have hindered the understanding of their physiological roles in specific subcellular compartments. Here we develop a method to rapidly deplete tubulin glutamlyation inside the primary cilia, a microtubule-based sensory organelle protruding on the cell surface, by targeting an engineered deglutamylase to the cilia in minutes. This rapid deglutamylation quickly leads to altered ciliary functions such as kinesin-2-mediated anterograde intraflagellar transport and Hedgehog signaling, along with no apparent crosstalk to other PTMs such as acetylation and detyrosination. Our study offers a feasible approach to spatiotemporally manipulate tubulin PTMs in living cells. Future expansion of the repertoire of actuators that regulate PTMs may facilitate a comprehensive understanding of how diverse tubulin PTMs encode ciliary as well as cellular functions.}, URL = {https://www.biorxiv.org/content/early/2018/03/15/282103}, eprint = {https://www.biorxiv.org/content/early/2018/03/15/282103.full.pdf}, journal = {bioRxiv} }