PT  - JOURNAL ARTICLE
AU  - Shi-Rong Hong
AU  - Cuei-Ling Wang
AU  - Yao-Shen Huang
AU  - Yu-Chen Chang
AU  - Ya-Chu Chang
AU  - Ganesh V. Pusapati
AU  - Chun-Yu Lin
AU  - Ning Hsu
AU  - Hsiao-Chi Cheng
AU  - Yueh-Chen Chiang
AU  - Wei-En Huang
AU  - Nathan C. Shaner
AU  - Rajat Rohatgi
AU  - Takanari Inoue
AU  - Yu-Chun Lin
TI  - Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling
AID  - 10.1101/282103
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 282103
4099  - http://biorxiv.org/content/early/2018/03/15/282103.short
4100  - http://biorxiv.org/content/early/2018/03/15/282103.full
AB  - Tubulin post-translational modifications (PTMs) occur spatiotemporally throughout cells and are suggested to be involved in a wide range of cellular activities. However, the complexity and dynamic distribution of tubulin PTMs within cells have hindered the understanding of their physiological roles in specific subcellular compartments. Here we develop a method to rapidly deplete tubulin glutamlyation inside the primary cilia, a microtubule-based sensory organelle protruding on the cell surface, by targeting an engineered deglutamylase to the cilia in minutes. This rapid deglutamylation quickly leads to altered ciliary functions such as kinesin-2-mediated anterograde intraflagellar transport and Hedgehog signaling, along with no apparent crosstalk to other PTMs such as acetylation and detyrosination. Our study offers a feasible approach to spatiotemporally manipulate tubulin PTMs in living cells. Future expansion of the repertoire of actuators that regulate PTMs may facilitate a comprehensive understanding of how diverse tubulin PTMs encode ciliary as well as cellular functions.