RT Journal Article
SR Electronic
T1 Modulation of sensory behavior and food choice by an enteric bacteria-produced neurotransmitter
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 735845
DO 10.1101/735845
A1 Michael P. O’Donnell
A1 Bennett W. Fox
A1 Pin-Hao Chao
A1 Frank C. Schroeder
A1 Piali Sengupta
YR 2019
UL http://biorxiv.org/content/early/2019/08/15/735845.abstract
AB Animals coexist in commensal, pathogenic or mutualistic relationships with complex communities of diverse organisms including microbes1. Some bacteria produce bioactive neurotransmitters which have been proposed to modulate host nervous system activity and behaviors2. However, the mechanistic basis of this microbiota-brain modulation and its physiological relevance is largely unknown. Here we show that in C. elegans, the neuromodulator tyramine (TA) produced by gut-colonizing commensal Providencia bacteria can bypass the requirement for host TA biosynthesis to manipulate a host sensory decision. Bacterially-produced TA is likely converted to octopamine (OA) by the host tyramine beta-hydroxylase enzyme. OA, in turn, targets the OCTR-1 receptor on the ASH/ASI sensory neurons to modulate an aversive olfactory response. We identify genes required for TA biosynthesis in Providencia, and show that these genes are necessary for modulation of host behavior. We further find that C. elegans colonized by Providencia preferentially select these bacteria in food choice assays, and that this selection bias requires bacterially-produced TA. Our results demonstrate that a neurotransmitter produced by gut microbiota mimics the functions of the cognate host molecule to override host control of a sensory decision, thereby promoting fitness of both host and microbe.