TY - JOUR T1 - Regulatory kinase genetic interaction profiles differ between environmental conditions and cellular states JF - bioRxiv DO - 10.1101/735720 SP - 735720 AU - Siyu Sun AU - Anastasia Baryshnikova AU - Nathan Brandt AU - David Gresham Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/15/735720.abstract N2 - Cell growth and quiescence in eukaryotic cells is controlled by an evolutionarily conserved network of signaling pathways. Signal transduction networks operate to modulate a wide range of cellular processes and physiological properties when cells exit proliferative growth and initiate a quiescent state. How signaling networks function to respond to diverse signals that result in cell cycle exit and establishment of a quiescent state is poorly understood. Here, we studied the function of signaling pathways in quiescent cells using global genetic interaction mapping in the model eukaryotic cell, Saccharomyces cerevisiae (budding yeast). We performed pooled analysis of genotypes using molecular barcode sequencing to test the role of ∼3,900 gene deletion mutants and ∼11,700 pairwise interactions between all non-essential genes and the protein kinases TOR1, RIM15, PHO85 in three different nutrient-restricted conditions in both proliferative and quiescent cells. We detect nearly five-fold more genetic interactions in quiescent cells compared to proliferative cells. We find that both individual gene effects and genetic interaction profiles vary depending on the specific pro-quiescence signal. The master regulator of quiescence, RIM15 shows distinct genetic interaction profiles in response to different starvation signals. However, vacuole-related functions show consistent genetic interactions with RIM15 in response to different starvation signals suggesting that RIM15 integrates diverse signals to maintain protein homeostasis in quiescent cells. Our study expands genome-wide genetic interaction profiling to additional conditions, and phenotypes, highlighting the conditional dependence of epistasis. ER -