PT - JOURNAL ARTICLE AU - Gilles Flouriot AU - Charly Jehanno AU - Yann le Page AU - Pascale le Goff AU - Benjamin Boutin AU - Denis Michel TI - The basal level of gene expression associated with chromatin loosening shapes Waddington landscapes and controls cell differentiation AID - 10.1101/738120 DP - 2019 Jan 01 TA - bioRxiv PG - 738120 4099 - http://biorxiv.org/content/early/2019/08/16/738120.short 4100 - http://biorxiv.org/content/early/2019/08/16/738120.full AB - The baseline level of transcription is variable and seriously complicates the normalization of comparative transcriptomic data, but its biological importance remains unappreciated. We show that this ingredient is in fact crucial for the interpretation of molecular biology results. It is correlated to the degree of chromatin loosening measured by DNA accessibility, and systematically leads to cellular dedifferentiation as assessed by transcriptomic signatures, irrespective of the molecular and cellular tools used. A theoretical analysis of gene circuits formally involved in differentiation, reveals that the epigenetic landscapes of Waddington are restructured by the level of non-specific expression, such that the attractors of progenitor and differentiated cells can be mutually exclusive. Together, these results unveil a generic principle of epigenetic landscape remodeling in which the basal gene expression level, notoriously important in pluripotent cells, allows the maintenance of stemness by generating a specific landscape and in turn, its reduction favors multistability and thereby differentiation. This study highlights how heterochromatin maintenance is essential for preventing pathological cellular reprogramming, age-related diseases and cancer.