RT Journal Article SR Electronic T1 G-quadruplex prediction in E.coli genome reveals a conserved putative G-quadruplex-Hairpin-Duplex switch JF bioRxiv FD Cold Spring Harbor Laboratory SP 032615 DO 10.1101/032615 A1 Osman Doluca A1 Oktay I Kaplan A1 Burak Berber A1 Nezih Hekim YR 2015 UL http://biorxiv.org/content/early/2015/11/23/032615.abstract AB Many studies show that short non-coding sequences are widely conserved among regulatory elements. More and more conserved sequences are being discovered since the development next generation sequencing technology. The common approach to identify conserved sequences with regulatory roles rely on the topological change such as hairpin formation on the DNA or RNA level. However G-quadruplexes, a non-canonical nucleic acid topology with little established biological role, is rarely considered for conserved regulatory element discovery. Here we present the use of G-quadruplex prediction algorithm to identify putative G-quadruplex-forming and conserved elements in E.coli genome. Phylogenetic analysis of 52 G-quadruplex forming sequences revealed two conserved G-quadruplex motifs with potential regulatory role.