RT Journal Article SR Electronic T1 DeepPoseKit: a software toolkit for fast and robust animal pose estimation using deep learning JF bioRxiv FD Cold Spring Harbor Laboratory SP 620245 DO 10.1101/620245 A1 Jacob M. Graving A1 Daniel Chae A1 Hemal Naik A1 Liang Li A1 Benjamin Koger A1 Blair R. Costelloe A1 Iain D. Couzin YR 2019 UL http://biorxiv.org/content/early/2019/08/20/620245.abstract AB Quantitative behavioral measurements are important for answering questions across scientific disciplines—from neuroscience to ecology. State-of-the-art deep-learning methods offer major advances in data quality and detail by allowing researchers to automatically estimate locations of an animal’s body parts directly from images or videos. However, currently-available animal pose estimation methods have limitations in speed and robustness. Here we introduce a new easy-to-use software toolkit, DeepPoseKit, that addresses these problems using an efficient multi-scale deep-learning model, called Stacked DenseNet, and a fast GPU-based peak-detection algorithm for estimating keypoint locations with subpixel precision. These advances improve processing speed >2× with no loss in accuracy compared to currently-available methods. We demonstrate the versatility of our methods with multiple challenging animal pose estimation tasks in laboratory and field settings—including groups of interacting individuals. Our work reduces barriers to using advanced tools for measuring behavior and has broad applicability across the behavioral sciences.