PT  - JOURNAL ARTICLE
AU  - Angus M. Sidore
AU  - Calin Plesa
AU  - Joyce A. Samson
AU  - Sriram Kosuri
TI  - DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
AID  - 10.1101/740977
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 740977
4099  - http://biorxiv.org/content/early/2019/08/20/740977.short
4100  - http://biorxiv.org/content/early/2019/08/20/740977.full
AB  - Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here we improve DropSynth, a low-cost, multiplexed method which builds gene libraries by compartmentalizing and assembling microarray-derived oligos in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction, and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.