RT Journal Article
SR Electronic
T1 DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 740977
DO 10.1101/740977
A1 Angus M. Sidore
A1 Calin Plesa
A1 Joyce A. Samson
A1 Sriram Kosuri
YR 2019
UL http://biorxiv.org/content/early/2019/08/20/740977.abstract
AB Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here we improve DropSynth, a low-cost, multiplexed method which builds gene libraries by compartmentalizing and assembling microarray-derived oligos in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction, and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.