TY - JOUR T1 - Structural Basis of H2B Ubiquitination-Dependent H3K4 Methylation by COMPASS JF - bioRxiv DO - 10.1101/740738 SP - 740738 AU - Peter L. Hsu AU - Hui Shi AU - Calvin Leonen AU - Jianming Kang AU - Champak Chatterjee AU - Ning Zheng Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/20/740738.abstract N2 - The COMPASS complex represents the prototype of the SET1/MLL family of methyltransferases that controls gene transcription by H3K4 methylation (H3K4me). Although H2B monoubiquitination (H2Bub) is well-known as a prerequisite histone mark for COMPASS activity, how the H2Bub-H3K4me crosstalk is catalyzed by COMPASS remains unclear. Here, we report the cryo-EM structures of an extended COMPASS catalytic module (CM) bound to the H2Bub and free nucleosome. The COMPASS CM clamps onto the nucleosome disk-face via an extensive interface to capture the flexible H3 N-terminal tail. The interface also sandwiches a critical Set1 arginine-rich motif (ARM) that auto-inhibits COMPASS. Unexpectedly, without enhancing COMPASS-nucleosome interaction, H2Bub activates the enzymatic assembly by packing against Swd1 and alleviating the inhibitory effect of the Set1 ARM upon fastening it to the acidic patch. By unmasking the spatial configuration of the COMPASS-H2Bub-nucleosome assembly, our studies establish the structural framework for understanding the long-studied H2Bub-H3K4me histone modification crosstalk. ER -