RT Journal Article
SR Electronic
T1 Establishment of signaling interactions with cellular resolution for every cell cycle of embryogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 285007
DO 10.1101/285007
A1 Chen, Long
A1 Ho, Vincy Wing Sze
A1 Wong, Ming-Kin
A1 Huang, Xiaotai
A1 Chan, Lu-yan
A1 Ng, Hon Chun Kaoru
A1 Ren, Xiaoliang
A1 Yan, Hong
A1 Zhao, Zhongying
YR 2018
UL http://biorxiv.org/content/early/2018/03/19/285007.abstract
AB Intercellular signaling interaction plays a key role in breaking fate symmetry during animal development. Identification of the signaling interaction at cellular resolution is technically challenging, especially in a developing embryo. Here we develop a platform that allows automated inference and validation of signaling interaction for every cell cycle of C. elegans embryogenesis. This is achieved by generation of a systems-level cell contact map that consists of 1,114 highly confident intercellular contacts by modeling analysis and is validated through cell membrane labeling coupled with cell lineage analysis. We apply the map to identify cell pairs between which a Notch signaling interaction takes place. By generating expression patterns for two ligands and two receptors of Notch signaling pathway with cellular resolution using automated expression profiling technique, we are able to refine existing and identify novel Notch interactions during C. elegans embryogenesis. Targeted cell ablation followed by cell lineage analysis demonstrates the roles of signaling interactions over cell division in breaking fate symmetry. We finally develop a website that allows online access to the cell-cell contact map for mapping of other signaling interaction in the community. The platform can be adapted to establish cellular interaction from any other signaling pathways.