RT Journal Article SR Electronic T1 Spread of the pandemic Zika virus lineage is associated with NS1 codon usage adaptation in humans JF bioRxiv FD Cold Spring Harbor Laboratory SP 032839 DO 10.1101/032839 A1 Caio César de Melo Freire A1 Atila Iamarino A1 Daniel Ferreira de Lima Neto A1 Amadou Alpha Sall A1 Paolo Marinho de Andrade Zanotto YR 2015 UL http://biorxiv.org/content/early/2015/11/25/032839.abstract AB Zika virus (ZIKV) infections were more common in the zoonotic cycle until the end of the 20th century with few human cases in Africa and Southeastern Asia. Recently, the Asian lineage of ZIKV is spreading along human-to-human chains of transmission in the Pacific Islands and in South America. To better understand its recent urban expansion, we compared genetic differences among the lineages. Herein we show that the recent Asian lineage spread is associated with significant NS1 codon usage adaptation to human housekeeping genes, which could facilitate viral replication and increase viral titers. These findings were supported by a significant correlation with growth in Malthusian fitness. Furthermore, we predicted several epitopes in the NS1 protein that are shared between ZIKV and Dengue. Our results imply in a significant dependence of the recent human ZIKV spread on NS1 translational selection.