PT  - JOURNAL ARTICLE
AU  - Rakesh Pathak
AU  - Priyanka Singh
AU  - Sudha Ananthakrishnan
AU  - Sarah Adamczyk
AU  - Olivia Schimmel
AU  - Chhabi K. Govind
TI  - Acetylation-Dependent Recruitment of the FACT Complex and Its Role in Regulating Pol II Occupancy Genome-Wide in <em>Saccharomyces cerevisiae</em>
AID  - 10.1101/285932
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 285932
4099  - http://biorxiv.org/content/early/2018/03/20/285932.short
4100  - http://biorxiv.org/content/early/2018/03/20/285932.full
AB  - Histone chaperones, chromatin remodelers, and histone modifying complexes play a critical role in alleviating the nucleosomal barrier for DNA-dependent processes. Here, we have examined the role of two highly conserved yeast (Saccharomyces cerevisiae) histone chaperones, FACT and Spt6, in regulating transcription. We show that the H3 tail contributes to the recruitment of FACT to coding sequences in a manner dependent on acetylation. We found that deleting a H3 HAT Gcn5 or mutating lysines on the H3 tail impairs FACT recruitment at ADH1 and ARG1 genes. However, deleting the H4 tail or mutating the H4 lysines failed to dampen FACT occupancy in coding regions. Additionally, we show that FACT-depletion reduces Pol II occupancy in the 5’ ends genome-wide. In contrast, Spt6-depletion leads to reduction in Pol II occupancy towards the 3’ end, in a manner dependent on the gene-length. Severe transcription and histone eviction defects were also observed in a strain that was impaired for Spt6 recruitment (spt6Δ202) and depleted of FACT. Importantly, the severity of the defect strongly correlated with WT Pol II occupancies at these genes, indicating critical roles of Spt6 and Spt16 in promoting high-level transcription. Collectively, our results show that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription.