RT Journal Article
SR Electronic
T1 Cellulose biosynthesis inhibition reduces cell cycle activity in a nitrate reductase- and cytokinin-dependent manner
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 286161
DO 10.1101/286161
A1 Gigli-Bisceglia, Nora
A1 Engelsdorf, Timo
A1 Strnad, Miroslav
A1 Vaahtera, Lauri
A1 Jamoune, Amel
A1 Alipanah, Leila
A1 Novák, Ondřej
A1 Hejatko, Jan
A1 Hamann, Thorsten
YR 2018
UL http://biorxiv.org/content/early/2018/03/21/286161.abstract
AB During growth, development and defense, cell wall integrity needs to be coordinated with cell cycle activity. In Saccharomyces cerevisiae, coordination is mediated by the cell wall integrity maintenance mechanism. In plants, little is known how coordination is achieved.Here we investigated coordination between plant cell wall and cell cycle activity in Arabidopsis thaliana seedlings by studying the impact of cell wall damage (CWD, caused by cellulose biosynthesis inhibition) on cell cycle gene expression, growth, phytohormone (jasmonic acid, salicylic acid, cytokinins) and lignin accumulation.We found root growth and cell cycle gene expression are reduced by CWD in an osmo-sensitive manner. trans-zeatin application suppressed the CWD effect on gene expression. Quantification of cytokinins revealed CWD-induced, osmo-sensitive changes in several cytokinins. Expression of CYTOKININ OXIDASE2/DEHYDROGENASE (CKX2) and CKX3, encoding cytokinin-degrading enzymes, was elevated in CWD-exposed seedlings. Genetic studies implicated NITRATE REDUCTASE1/2 (NIA1/2) in the response to CWD. In nia1/2 seedlings CWD induced neither expression of CKX2/3 and cell cycle genes nor accumulation of jasmonic acid, salicylic acid and lignin.This suggests that CWD causes increased CKX2/3 expression through a NIA1/2-mediated process. Increased CKX expression seems to cause changes in cytokinin levels, leading to reduced cell cycle gene expression.