RT Journal Article
SR Electronic
T1 The <em>Setaria viridis</em> genome and diversity panel enables discovery of a novel domestication gene
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 744557
DO 10.1101/744557
A1 Pu Huang
A1 Sujan Mamidi
A1 Adam Healey
A1 Jane Grimwood
A1 Jerry Jenkins
A1 Kerrie Barry
A1 Avinash Sreedasyam
A1 Shengqiang Shu
A1 Maximilian Feldman
A1 Jinxia Wu
A1 Yunqing Yu
A1 Cindy Chen
A1 Jenifer Johnson
A1 Hitoshi Sakakibara
A1 Takatoshi Kiba
A1 Tetsuya Sakurai
A1 Daniel Rokhsar
A1 Ivan Baxter
A1 Jeremy Schmutz
A1 Thomas P. Brutnell
A1 Elizabeth A. Kellogg
YR 2019
UL http://biorxiv.org/content/early/2019/08/24/744557.abstract
AB We present a platinum-quality genome assembly for the model grass Setaria viridis, and high quality genomic sequences of 600+ wild accessions (average 42.6x coverage). Presence-absence variation (PAV) and single-nucleotide polymorphisms (SNPs) identify several subpopulations in North America. Using genome-wide association mapping plus CRISPR-Cas9 technology, we identified and validated Less Shattering1 (SvLES1), a gene for seed shattering with a retrotransposon insertion in the domesticated S. italica (foxtail millet) allele. We also identified a candidate gene for erect leaves, orthologous to the maize gene liguleless2. These results demonstrate the utility of the model plant S. viridis for complex trait dissection in panicoid crops.