PT  - JOURNAL ARTICLE
AU  - Laura Ferrer-Font
AU  - Palak Mehta
AU  - Phoebe Harmos
AU  - Alfonso Schmidt
AU  - Kylie M Price
AU  - Ian F Hermans
AU  - Franca Ronchese
AU  - Graham Le Gros
AU  - Johannes U Mayer
TI  - Single-cell analysis of intestinal immune cells during helminth infection
AID  - 10.1101/746479
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 746479
4099  - http://biorxiv.org/content/early/2019/08/24/746479.short
4100  - http://biorxiv.org/content/early/2019/08/24/746479.full
AB  - Single cell isolation from helminth infected intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion protocol, we were able to isolate millions of immune cells from heavily infected tissues. Using this protocol, we validated many hallmarks of anti-parasite immunity and analyzed immune cells from the lamina propria and granulomas during helminth development, as well as acute and chronic worm infection.