PT  - JOURNAL ARTICLE
AU  - J.P. Fuenzalida
AU  - T. Xiong
AU  - B. M. Moerschbacher
AU  - Marc Ostermeier
AU  - F.M. Goycoolea
TI  - Protein-surfactant-polysaccharide nanoparticles increase the catalytic activity of an engineered β-lactamase maltose-activated switch enzyme
AID  - 10.1101/746560
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 746560
4099  - http://biorxiv.org/content/early/2019/08/24/746560.short
4100  - http://biorxiv.org/content/early/2019/08/24/746560.full
AB  - We present polysaccharide-based nanoparticles able to associate and increase the catalytic activity of the maltose-binding MBP317-347 switch enzyme. Fluorescence quenching and molecular docking studies along with the partial resistance to increasing pH and ionic strength indicate that the increase in enzymatic activity is due to a specific interaction between the maltose binding pocket on MBP317-347 and alginate exposed on the surface of the nanoparticles. Finally, we show that the hybrid self co-assembled particles increase the half-life of MBP317-347 over six-fold at 37°C, thus reflecting their potential use as a macromolecular drug delivery system.