PT  - JOURNAL ARTICLE
AU  - Joan Mas-Lloret
AU  - Mireia Obón-Santacana
AU  - Gemma Ibáñez-Sanz
AU  - Elisabet Guinó
AU  - Miguel L Pato
AU  - Francisco Rodriguez-Moranta
AU  - Alfredo Mata
AU  - Ana García-Rodríguez
AU  - Victor Moreno
AU  - Ville Nikolai Pimenoff
TI  - Gut microbiome diversity detected by high-coverage 16S and shotgun sequencing of matched stool and colon biopsy samples
AID  - 10.1101/742635
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 742635
4099  - http://biorxiv.org/content/early/2019/08/25/742635.short
4100  - http://biorxiv.org/content/early/2019/08/25/742635.full
AB  - The gut microbiome has a fundamental role in human health and disease. However, studying the complex structure and function of the gut microbiome using next generation sequencing is challenging and prone to reproducibility problems due to the heterogeneity of sample sets. Here, we obtained cross-sectional colon biopsies and faecal samples from nine participants in our COLSCREEN study and sequenced them in high coverage using Illumina pair-end shotgun (for faecal samples) and IonTorrent 16S (for paired feces and colon biopsies) technologies. The metagenomes consisted of between 47 and 92 million reads per sample and the targeted sequencing covered more than 300K reads per sample across seven hypervariable regions of the 16S gene. Our data is freely available and coupled with code for the presented metagenomic analysis using up-to-date bioinformatics algorithms. These results will add up to the informed insights into designing comprehensive microbiome analysis and also provide data for further testing for unambiguous gut microbiome analysis.