PT  - JOURNAL ARTICLE
AU  - Prashant Sinha
AU  - Pragya Jaiswal
AU  - Ashwin K. Jainarayanan
AU  - Samir K. Brahmachari
TI  - Intronic miRNA Mediated Gene Expression Regulation Controls Protein Crowding Inside the Cell
AID  - 10.1101/286906
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 286906
4099  - http://biorxiv.org/content/early/2018/03/22/286906.short
4100  - http://biorxiv.org/content/early/2018/03/22/286906.full
AB  - SUMMARY Gene regulatory effects of microRNAs at a posttranscriptional level has been established over the last decade. In this study, we analyze the interaction networks of mRNA translation regulation through intronic miRNA, under various tissue-specific cellular contexts, taking into account the thermodynamic affinity, kinetics, and the presence of competitive interactors. This database, and analysis has been made available through an open-access web-server, miRiam, to promote further exploration.Here we report that expression of genes involved in Apoptosis Processes, Immune System Processes, Translation Regulator Activities, and Molecular Transport Activities within the cell are predominately regulated by miRNA mediation. Our findings further indicate that this regulatory effect has a profound effect in controlling protein crowding inside the cell. A miRNA mediated gene expression regulation serves as a temporal regulator, allowing the cellular machinery to temporarily ‘pause’ the translation of mRNA, indicating that the miRNA–mRNA interactions may be important for governing the optimal usage of cell volume.