PT - JOURNAL ARTICLE AU - Shengdong Luo AU - Zemin He AU - Zhihui Sun AU - Yonghui Yu AU - Yongqiang Jiang AU - Yigang Tong AU - Lihua Song TI - Culture under normoxic conditions and enhanced virulence of phase II <em>Coxiella burnetii</em> transformed with a RSF1010-based shuttle vector AID - 10.1101/747220 DP - 2019 Jan 01 TA - bioRxiv PG - 747220 4099 - http://biorxiv.org/content/early/2019/08/28/747220.short 4100 - http://biorxiv.org/content/early/2019/08/28/747220.full AB - Coxiella burnetii is a Gram-negative, facultative intracellular microorganism that can cause acute or chronic Q fever in human. It was recognized as an obligate intracellular organism until the revolutionary design of an axenic cystine culture medium (ACCM). Present axenic culture of C. burnetii strictly requires a hypoxic condition (&lt;10% oxygen). Here we investigated the normoxic growth of C. burnetii strains in ACCM-2 with or without tryptophan supplementation. Three C. burnetii strains - Henzerling phase I, Nine Mile phase II and a Nine Mile phase II transformant, were included. The transformant contains a pMMGK plasmid that is composed of a RSF1010 ori, a repABC operon, an eGFP gene and a kanamycin resistance cassette. We found that, under normoxia if staring from an appropriate concentration of fresh age inocula, Nine Mile phase II can grow significantly in ACCM-2 with tryptophan, while the transformant can grow robustly in ACCM-2 with or without tryptophan. In contrast, long-term frozen stocks of phase II and its transformant, and Henzerling phase I of different ages had no growth capability under normoxia under any circumstances. Furthermore, frozen stocks of the transformant consistently caused large splenomegaly in SCID mice, while wild type Nine Mile phase II induced a lesser extent of splenomegaly. Taken together, our data show that normoxic cultivation of phase II C. burnetii can be achieved under certain conditions. Our data suggests that tryptophan and an unknown temperature sensitive signal are involved in the expression of genes for normoxic growth regulated by quorum sensing in C. burnetii.