RT Journal Article
SR Electronic
T1 A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 750083
DO 10.1101/750083
A1 Eszter Toth
A1 Sayed Muhammed Rassul
A1 Martin Berry
A1 Daniel Fulton
YR 2019
UL http://biorxiv.org/content/early/2019/08/29/750083.abstract
AB Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinating OL would advance our understanding of myelin plasticity and injury, thus we adapted a labelling approach involving Semliki Forest Virus (SFV) vectors to resolve and quantify the 3-D structure of OL processes and internodes in cerebellar slice cultures. We first demonstrate the utility of the approach by studying changes in OL morphology after complement-mediated injury. SFV vectors injected into cerebellar white matter labelled transitional OL (TOL), whose characteristic mixture of myelinating and non-myelinating processes exhibited significant degeneration after complement injury. The method was also capable of resolving finer changes in morphology related to neuronal activity. Prolonged suppression of neuronal activity, which reduced myelination, increased the number of TOL processes, while decreasing both the length of putative internodes, and the proportion of myelinating terminal branches. Overall this approach provides novel information on the morphology of TOL, and new opportunities to study the response of OL to conditions that alter circuit function or induce demyelination.