RT Journal Article
SR Electronic
T1 High-throughput sequencing analysis of bacterial diversity in raw and pasteurized goat milk
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 751149
DO 10.1101/751149
A1 Feng Huang
A1 Siqi Liu
A1 Xiaokang Zhou
A1 Pengfei Wang
A1 Rengchun He
A1 Zhiyang Zhou
A1 Caixia Zou
YR 2019
UL http://biorxiv.org/content/early/2019/08/29/751149.abstract
AB The aim of this study was to evaluate the microbial composition of both raw and pasteurized goat milk using high-throughput DNA sequencing. This analysis revealed that the dominant phylum found in the raw milk was Proteobacteria, and the dominant genus was Kluyvera; Proteobacteria and Kluyvera constituted up to 67.66% and 28.85% of the total bacteria population, respectively. The microorganisms in goat milk predominantly consist of Gram-negative bacteria. Notably, Akkermansia and Faecalibacterium were identified in goat milk for the first time. In addition, the results also indicate that some bacteria in pasteurized goat milk may exist in a viable but nonculturable (VBNC) state. This study provides a theoretical basis that may aid the community in better understanding bacterial diversity in goat milk. The results of this study will help us to improve the quality and safety of goat milk.Importance The microbial diversity in goat milk and pasteurized goat milk at different refrigeration stages was described. Several bacterial species that have not previously been reported in goat milk were identified, including many VBNC bacteria. The findings provided the necessary microbial information for quality and safety of goat milk and dairy products.