PT  - JOURNAL ARTICLE
AU  - Mohamadreza Fazel
AU  - Michael J. Wester
AU  - Bernd Rieger
AU  - Ralf Jungmann
AU  - Keith A. Lidke
TI  - Sub-Nanometer Precision using Bayesian Grouping of Localizations
AID  - 10.1101/752287
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 752287
4099  - http://biorxiv.org/content/early/2019/08/30/752287.short
4100  - http://biorxiv.org/content/early/2019/08/30/752287.full
AB  - Single-molecule localization microscopy super-resolution methods such as DNA-PAINT and (d)STORM can generate multiple observed localizations over the time course of data acquisition from each dye or binding site that are not a priori assigned to those specific dyes or binding sites. We describe a Bayesian method of grouping and combining localizations from multiple blinking/binding events that can improve localization precision to better than one nanometer. The known statistical distribution of the number of binding/blinking events per dye/docking strand along with the precision of each localization event are used to estimate the true number and location of emitters in closely-spaced clusters.