RT Journal Article
SR Electronic
T1 Sub-Nanometer Precision using Bayesian Grouping of Localizations
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 752287
DO 10.1101/752287
A1 Mohamadreza Fazel
A1 Michael J. Wester
A1 Bernd Rieger
A1 Ralf Jungmann
A1 Keith A. Lidke
YR 2019
UL http://biorxiv.org/content/early/2019/08/30/752287.abstract
AB Single-molecule localization microscopy super-resolution methods such as DNA-PAINT and (d)STORM can generate multiple observed localizations over the time course of data acquisition from each dye or binding site that are not a priori assigned to those specific dyes or binding sites. We describe a Bayesian method of grouping and combining localizations from multiple blinking/binding events that can improve localization precision to better than one nanometer. The known statistical distribution of the number of binding/blinking events per dye/docking strand along with the precision of each localization event are used to estimate the true number and location of emitters in closely-spaced clusters.