TY - JOUR T1 - Sub-Nanometer Precision using Bayesian Grouping of Localizations JF - bioRxiv DO - 10.1101/752287 SP - 752287 AU - Mohamadreza Fazel AU - Michael J. Wester AU - Bernd Rieger AU - Ralf Jungmann AU - Keith A. Lidke Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/08/30/752287.abstract N2 - Single-molecule localization microscopy super-resolution methods such as DNA-PAINT and (d)STORM can generate multiple observed localizations over the time course of data acquisition from each dye or binding site that are not a priori assigned to those specific dyes or binding sites. We describe a Bayesian method of grouping and combining localizations from multiple blinking/binding events that can improve localization precision to better than one nanometer. The known statistical distribution of the number of binding/blinking events per dye/docking strand along with the precision of each localization event are used to estimate the true number and location of emitters in closely-spaced clusters. ER -