RT Journal Article
SR Electronic
T1 Spatially clustered loci with multiple enhancers are frequent targets of HIV-1
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 287896
DO 10.1101/287896
A1 Lucic, Bojana
A1 Chen, Heng-Chang
A1 Kuzman, Maja
A1 Zorita, Eduard
A1 Wegner, Julia
A1 Minneker, Vera
A1 Roukos, Vassilis
A1 Wang, Wei
A1 Fronza, Raffaele
A1 Schmidt, Manfred
A1 Benkirane, Monsef
A1 Stadhouders, Ralph
A1 Vlahovicek, Kristian
A1 Filion, Guillaume J
A1 Lusic, Marina
YR 2018
UL http://biorxiv.org/content/early/2018/03/24/287896.abstract
AB HIV-1 recurrently targets active genes that are positioned in the outer shell of the nucleus and integrates in the proximity of the nuclear pore compartment. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are delineated with super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location at the nuclear periphery during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results show for the first time the relevance of the spatial compartmentalization of the genome for HIV-1 integration, thus further strengthening the role of nuclear architecture in viral infection.