PT  - JOURNAL ARTICLE
AU  - Denis Krndija
AU  - Michael Fairhead
TI  - IGF1R undergoes active and directed centripetal transport on filopodia upon receptor activation
AID  - 10.1101/754960
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 754960
4099  - http://biorxiv.org/content/early/2019/09/02/754960.short
4100  - http://biorxiv.org/content/early/2019/09/02/754960.full
AB  - Filopodia are thin, actin-based membrane protrusions with roles in sensing external mechanical and chemical cues, such as growth factor gradients in tissues. It was proposed that the chemical sensing role of filopodia is achieved through clearance of activated signaling receptors from filopodia. Type I insulin-like growth factor receptor (IGF1R) is a key regulator of normal development and growth, as well as tumor development and progression. Its biological roles depend on its activation upon IGF1 binding at the cell membrane. IGF1R behavior at the cell membrane and in particular in filopodia, has not been established. We found that IGF1 activation led to a gradual reduction in IGF1R puncta in filopodia, and that this clearance depended on actin, non-muscle myosin II, and IGF1R kinase activity. Using single particle tracking of filopodial IGF1R, we established that ligand-free IGF1R undergoes non-directional unidimensional diffusion along the filopodium. Moreover, after initial diffusion, the ligand-bound IGF1R is actively transported along the filopodium towards the filopodium base, and consequently cleared from the filopodium. Our results show that IGF1R can move directionally on the plasma membrane protrusions, supporting a sensory role for filopodia in interpreting local IGF1 gradients.