RT Journal Article
SR Electronic
T1 The Influence of Blood Collection Tubes in Biomarkers Screening by Mass Spectrometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 753111
DO 10.1101/753111
A1 Siyuan Zhang
A1 Zixuan Zhao
A1 Wenjing Duan
A1 Zhaoxin li
A1 Zhuhui Nan
A1 Hanzhi Du
A1 Mengchang Wang
A1 Juan Yang
A1 Chen Huang
YR 2019
UL http://biorxiv.org/content/early/2019/09/04/753111.abstract
AB Background Mass spectrometry (MS) is one of the rapidly developing bio-analytical techniques in recent years and have found many biomarkers of variety of disease. Whereas pre-analytical process is one of most crucial procedure which would significantly influence the results of biomarkers screening. In the current study, we conducted a pilot analysis of serum to determine the effects of blood collection tubes in biomarkers screening.Methods Magnetic bead separation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry were used for qualitative analysis of healthy control and serum cancer patients. A total of 24 serum samples were analyzed in this study, of which were collected from patients or healthy control using non-additive tubes or coagulant activator tubes respectively. ClinProTools were used to compare the difference among the different groups.Results The results demonstrated that no matter for patients or normal people, the serum protein profile changed significantly when using coagulant tubes. We also found that the effect of coagulant on serum protein of patients was smaller than that of control group. There were significant differences among 27 peaks which were obtained in the control group and the control coagulant group. However, between patient group and patient coagulant group, only 1 differential peak were obtained. Coagulant changed the protein expression difference in the original serum, and the difference expanded, narrowed even reversed, most of which are small polypeptides (Mass<3000 Da), which significantly changed the results of biomarkers screening. The results showed that 19 potential biomarkers could be found with non-additive tubes and 16 potential biomarkers could be found with coagulate activator tubes, among which only 6 were the same.Conclusions The choice of blood collection tube significantly influence the results of biomarkers screening by MS.