RT Journal Article
SR Electronic
T1 Computational exploration of molecular receptive fields in the olfactory bulb reveals a glomerulus-centric chemical map
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 489666
DO 10.1101/489666
A1 Soelter, Jan
A1 Schumacher, Jan
A1 Spors, Hartwig
A1 Schmuker, Michael
YR 2019
UL http://biorxiv.org/content/early/2019/09/04/489666.abstract
AB Progress in olfactory research is currently hampered by incomplete knowledge about chemical receptive ranges of primary receptors. Moreover, the chemical logic underlying the arrangement of computational units in the olfactory bulb has still not been resolved. We undertook a large-scale approach at characterising molecular receptive ranges (MRRs) of glomeruli innervated by the MOR18-2 olfactory receptor in the dorsal olfactory bulb (dOB). Guided by an iterative approach that combined biological screening and machine learning, we selected 214 odorants to characterise the response of MOR18-2 and its neighbouring glomeruli. We discovered several previously unknown odorants activating MOR18-2 glomeruli, and we obtained detailed MRRs of MOR18-2 glomeruli and their neighbours. Physico-chemical MRR descriptions revealed that the spatial layout of glomeruli followed a chemical logic. Our results confirm earlier findings that demonstrate a partial chemical map underlying glomerular arrangement in the dOB. Moreover, our novel methodology that combines machine learning and physiological measurements lights the way towards future high-throughput studies to deorphanise and characterise structure-activity relationships in olfaction.