PT - JOURNAL ARTICLE AU - Amy Bergmann AU - Katherine Floyd AU - Melanie Key AU - Carly Dameron AU - Kerrick C. Rees AU - Daniel C. Whitehead AU - Iqbal Hamza AU - Zhicheng Dou TI - <em>Toxoplasma gondii</em> requires its plant-like heme biosynthesis pathway for infection AID - 10.1101/753863 DP - 2019 Jan 01 TA - bioRxiv PG - 753863 4099 - http://biorxiv.org/content/early/2019/09/06/753863.short 4100 - http://biorxiv.org/content/early/2019/09/06/753863.full AB - Heme, an iron-enclosed organic ring, is essential for virtually all living organisms by serving as a prosthetic group in proteins that function in diverse cellular activities ranging from diatomic gas transport and detection to mitochondrial respiration to detoxification. Cellular heme levels in microbial pathogens can be a composite of endogenous de novo synthesis or exogenous uptake of heme or heme synthesis intermediates1,2. Intracellular pathogenic microbes switch routes for heme supply when heme availability in their replicative environment fluctuates through infections2. Here, we show that the Toxoplasma gondii, an obligate intracellular human pathogen, encodes a functional heme biosynthesis pathway. A chloroplast-derived organelle, termed apicoplast, is involved in the heme production. Genetic and chemical manipulation revealed that de novo heme production is essential for T. gondii intracellular growth and pathogenesis. Surprisingly, the herbicide oxadiazon significantly impaired Toxoplasma growth, consistent with phylogenetic analyses that show T. gondii protoporphyrinogen oxidase is more closely related to plants than mammals. We further improve upon this inhibition by 15- to 25-fold with two oxadiazon derivatives, providing therapeutic proof that Toxoplasma heme biosynthesis is a druggable target. As T. gondii has been used to model other apicomplexan parasites3, our study underscores the utility of targeting heme biosynthesis in other pathogenic apicomplexans.