RT Journal Article
SR Electronic
T1 In vitro comparison of the internal ribosomal entry site activity from rodent hepacivirus and pegivirus
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 761379
DO 10.1101/761379
A1 Sims, Stuart
A1 Michaelsen, Kevin
A1 Burkhard, Sara
A1 Fraefel, Cornel
YR 2019
UL http://biorxiv.org/content/early/2019/09/08/761379.abstract
AB The 5’ untranslated region (5’ UTR) of rodent hepacivirus (RHV) and pegivirus (RPgV) contain sequence homology to the HCV type IV internal ribosome entry sites (IRES). Utilizing a monocistronic expression vector with an RNA polymerase I promoter to drive transcription we show cell-specific IRES translation and regions within the IRES required for full functionality. Focusing on RHV we further pseudotyped lentivirus with RHV and showed cell surface expression of the envelope proteins and transduction of murine hepatocytes.