RT Journal Article
SR Electronic
T1 Sessile growth reveals novel paradigms of <em>Pseudomonas aeruginosa</em> iron-regulated antimicrobial activity against <em>Staphylococcus aureus</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 765115
DO 10.1101/765115
A1 Luke K. Brewer
A1 Weiliang Huang
A1 Maureen A. Kane
A1 Amanda G. Oglesby-Sherrouse
YR 2019
UL http://biorxiv.org/content/early/2019/09/11/765115.abstract
AB Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens that cause chronic, polymicrobial infections. Each pathogen possesses a complex regulatory network that modulates iron acquisition and virulence. However, our current knowledge of these networks is largely based on studies with shaking cultures, which are not likely representative of microbial communities in vivo. Here, we provide proteomic, metabolic, and genetic evidence that iron regulation is altered in sessile P. aeruginosa cultures. We further demonstrate that iron-regulated interactions between P. aeruginosa and S. aureus are mediated by distinct factors in shaking versus sessile bacterial cultures. Moreover, we identified type 6 secretion as a target of iron regulation in P. aeruginosa in static but not shaking conditions, and co-culture studies suggest this system may contribute to antimicrobial activity against S. aureus in static conditions. These results yield new bacterial iron regulation paradigms and highlight the need for re-defining iron homeostasis in sessile microbial communities.