RT Journal Article
SR Electronic
T1 Integrated clonal analysis reveals circulating tumor DNA in urine and plasma of glioma patients
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 758441
DO 10.1101/758441
A1 Florent Mouliere
A1 Katrin Heider
A1 Christopher G. Smith
A1 Jing Su
A1 Mareike Thompson
A1 James Morris
A1 Jonathan C.M. Wan
A1 Dineika Chandrananda
A1 James Hadfield
A1 Marta Grezlak
A1 Irena Hudecova
A1 Wendy Cooper
A1 Davina Gale
A1 Matt Eldridge
A1 Colin Watts
A1 Kevin Brindle
A1 Nitzan Rosenfeld
A1 Richard Mair
YR 2019
UL http://biorxiv.org/content/early/2019/09/11/758441.abstract
AB Glioma-derived cell-free tumor DNA is challenging to detect using standard liquid biopsy techniques as its levels in body fluids are very low, similar to those in patients with early stage carcinomas. By sequencing cell-free DNA across thousands of clonal and private mutations identified individually in each patient’s tumor we detected tumor-derived DNA in plasma (10/12, 83%) and urine samples (8/11, 72%) from the majority (7/8, 87.5%) of glioma patients tested.One Sentence Summary Circulating tumor DNA can be detected in the majority of plasma and urine samples from primary brain tumor patients using sequencing guided by mutations detected in multi-region tumor biopsies.