RT Journal Article
SR Electronic
T1 ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 763714
DO 10.1101/763714
A1 Ekasit Sonpho
A1 Chanida Wootthichairangsan
A1 Miyuki Ishida
A1 Takeshi Inoue
A1 Kiyokazu Agata
A1 Anchuleerat Maleehuan
A1 Komgrid Charngkaew
A1 Nusara Chomanee
A1 Saengduen Moonsom
A1 Patompon Wongtrakoongate
A1 Arthit Chairoungdua
A1 Puey Ounjai
YR 2019
UL http://biorxiv.org/content/early/2019/09/11/763714.abstract
AB Extracellular matrix (ECM) plays key roles in shaping fates of stem cells, not only by providing suitable niche but also by mediating physical and biochemical cues. Despite intensive investigations on regeneration, the roles of ECM on fate determination of stem cells in animal with great regenerative potency, such as planarian, remained unclear. Here, we developed a method to decellularizing and isolating extracellular matrix from planarians. Although the isolated scaffold appears translucent, it contains all the internal features, resembling the structure of intact planarian, and which we thus called “ECM-body”. Nuclear staining demonstrated that ECM-body contains very little or no cell remained. Histological sections displayed a well-preserved morphological integrity of the specimen. Scanning electron microscope showed porous surface on ECM-body, potentially suitable for housing cells. Furthermore, our preliminary experiment suggested that ECM-body can be utilized as biomimetic scaffold for cell culture as it may support survival of injected neoblasts.