PT - JOURNAL ARTICLE AU - Marija Matejčić AU - Guillaume Salbreux AU - Caren Norden TI - A non-cell autonomous actin redistribution enables isotropic retinal growth AID - 10.1101/293951 DP - 2018 Jan 01 TA - bioRxiv PG - 293951 4099 - http://biorxiv.org/content/early/2018/04/03/293951.short 4100 - http://biorxiv.org/content/early/2018/04/03/293951.full AB - Tissue shape is often established early in development and needs to be scaled isotropically during growth. However, the cellular contributors and ways in which cells interact inside tissues to enable coordinated isotropic tissue scaling are not yet understood. Here, we follow cell and tissue shape changes in the zebrafish retinal neuroepithelium, which forms a cup with a smooth surface early in development and maintains this architecture as it grows. By combining 3D analysis and theory, we show that a global increase in cell height is necessary to maintain this tissue shape during growth. Timely cell height increase is governed by non-cell autonomous actin redistribution. Blocking actin redistribution and cell height increase perturbs isotropic scaling and leads to disturbed, folded tissue shape. Taken together, our data show how global changes in cell shape enable isotropic growth of the developing retinal neuroepithelium, a concept that could also apply to other systems.AbbreviationsCoVCoefficient of VariationECMextracellular matrixhdac1histone-deacetylase 1hpfhours post fertilizationpH3phosphorylated histone H3PSEpseudostratified epithelium