PT  - JOURNAL ARTICLE
AU  - T. Sansen
AU  - D. Sanchez-Fuentes
AU  - R. Rathar
AU  - A. Colom-Diego
AU  - F. El Alaoui
AU  - S. de Rossi
AU  - J. Viaud
AU  - M. Macchione
AU  - S. Matile
AU  - R. Gaudin
AU  - A. Carretero-Genevrier
AU  - L. Picas
TI  - Nanoscale topography templates the organization of stable clathrin/AP-2 structures
AID  - 10.1101/767590
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 767590
4099  - http://biorxiv.org/content/early/2019/09/12/767590.short
4100  - http://biorxiv.org/content/early/2019/09/12/767590.full
AB  - Eukaryotic cells are constantly submitted to shape changes as a result of fundamental cellular processes such as cell differentiation, migration or division, but also as a response to the extracellular environment. Previous studies point out to the plasma membrane curvature as a major mechanosensing mechanism1,2, although other processes might participate in the biochemical transduction of topographical cues3. Thus, how cells sense and respond to the external topography is not well understood. A bottleneck to address this question demands to conciliate the time-consuming and limited access to top-down nanofabrication techniques with cell biology and advance microscopy approaches. Here, we have engineered 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance coverslips by soft-gel nanoimprint lithography (soft-NIL)4, which is a cost-effective, customizable, large-scale fabrication and benchtop equipment-based method. By this novel fabrication of nanostructured substrates, we were able to perform super-resolution microscopy and to demonstrate that large membrane morphologies favor the formation of stable clathrin/AP-2 structures, a process that is assisted by the formation of functional actin networks.