PT  - JOURNAL ARTICLE
AU  - Kamel Jabbari
AU  - Johannes Wirtz
AU  - Martina Rauscher
AU  - Thomas Wiehe
TI  - Interdependence of linkage disequilibrium, chromatin architecture and compositional genome organization of mammals
AID  - 10.1101/293837
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 293837
4099  - http://biorxiv.org/content/early/2018/04/04/293837.short
4100  - http://biorxiv.org/content/early/2018/04/04/293837.full
AB  - Analysing chromatin architecture in interphase nuclei and recombination maps of human and mouse, we observed that blocks of elevated linkage disequilibrium tends to coincide with topologically associated domains (TADs) and isochores. There is a strong correlation between the GC level of TADs, double strand break (DSB) frequency and the local recombination rate. In particular, cold and hot spots of recombination tend to fall in AT- and GC-rich TADs, respectively. Also, binding of proteins which are critical for meiotic recombination hot spots (PRMD9, Spo11, DMC1 and H3K4me3) is positively correlated with the GC level of TADs. We conclude that the occurrence of meiotic DSB and recombination is associated with the same (com)positional features that constrain the architecture of chromosomes in the interphase nucleus of progenitor germ cells or pre-leptotene spermatocytes. This raises the possibility that regional variation of recombination is defined by compositional and epigenetic factors underlying chromatin architecture.